Structural biology revolution led by technical breakthroughs in cryo-electron microscopy

• 来源期刊: 中国物理B（英文版）
• 关键词: cryo-electron microscopy (cryo-EM) structural biology protein molecular machinery
• 年，卷（期）: 2018,（5）
• 页码范围: 49-58
• 页数: 10页
• 语种: 英文
• DOI: 10.1088/1674-1056/27/5/058703

摘要

Recent technical breakthroughs in cryo-electron microscopy (cryo-EM) revolutionized structural biology,which led to the 2017 Nobel Prize in chemistry being awarded to three scientists,Jacques Dubochet,Joachim Frank,and Richard Henderson,who made groundbreaking contributions to the development of cryo-EM.In this review,I will give a comprehensive review of the developmental history of cryo-EM,the technical aspects of the breakthrough in cryo-EM leading to the structural biology revolution,including electron microscopy,image recording devices and image processing algorithms,and the major scientific achievements by Chinese researchers employing cryo-EM,covering protein complexes involved in or related to gene expression and regulation,protein synthesis and degradation,membrane proteins,immunity,and viruses.Finally,I will give a perspective outlook on the development of cryo-EM in the future.