Fluorescence Spectroscopy and Molecular Docking Approach to Probe the Interaction between Dehydroeburicoic Acid and Human Serum Albumin

• 作者: Haiying Bao Rui Zhang Shijie Zheng Shude Yang Tolgor Bau Xianhao Cheng Yu Li
• 来源期刊: 微生物学(英文)
• 学科: 化学
• 关键词: Human Serum ALBUMIN Dehydroeburicoic ACID Interaction FLUORESCENCE Molecular DOCKING
• 年，卷（期）: 2019,（1）
• 页码范围: 21-37
• 页数: 17页
• 分类号: O6

摘要

The interaction between dehydroeburicoic acid (DeEA), a triterpene purified from medicinal fungi and the major transport protein, human serum albumin (HSA), were systematically studied by fluorescence spectroscopy, synchronous fluorescence spectroscopy, three-dimensional fluorescence spectroscopy and molecular docking approach under simulated physiological conditions. The intrinsic fluorescence of HSA was quenched through the combination of static and dynamic quenching mechanism. DeEA cannot be stored and carried by HSA in the body at higher temperature. The hydrogen bonding, hydrophobic force and van der Waals force were major acting forces. The site II was the major binding site. The energy transfer could occur with high probability and the binding distance was 3.29 nm. The binding process slightly changed the conformation and microenvironment of HSA. The DeEA molecule entered the hydrophobic cleft of HSA and formed the hydrogen bonding with Glu-492 and Lys-545.