基本信息来源于合作网站,原文需代理用户跳转至来源网站获取       
摘要:
Today,prognosis,diagnosis and treatment of cancers are progressing with non-invasive methods,including investigation and modification of the DNA methylation profile in cancer cells.One of the effective factors in regulating gene expression in mammals is DNA methylation.Methylation alterations of genes by external factors can change the expression of genes and inhibit the cancer.In the present study,we investigated the effect of Down syndrome critical region 1 gene(DSCR1)ectopic expression on the methylation status of the BCL-XL,ITGA6,TCF3,RASSF1A,DOK7,VIM and CXCR4 genes in breast cancer cell lines.The effect of DSCR1 ectopic expression on cell viability in MCF7,MDA-MB-468,MDA-MB-231 and MCF10A cell lines was evaluated using MTT assay after the cells treated by lentivirus vectors harboring DSCR1 for 72 hours.Methylation status of BCL-XL,ITGA6,TCF3,RASSF1A,DOK7,VIM and CXCR4 genes in breast cancer cell lines was assessed by Restriction Enzyme PCR(REP)method.Also,methylation changes of these genes in breast cancer cell lines after treatment by lentivirus vectors harboring DSCR1 for 7 days were analyzed by REP method.To confirm the effect of DSCR1 on methylation of genes,Real-time PCR was performed.The MTT assay results indicated that DSCR1 ectopic expression reduced cell viability in all three human breast cancer cell lines.Our results showed that DSCR1 ectopic expression after 6 days reversed the hypomethylation status of the BCLXL,ITGA6,TCF3,VIM and CXCR4 genes and hypermethylation of RASSF1A and DOK7 genes.The expression levels of BCL-XL,ITGA6,TCF3,VIM and CXCR4 mRNA significantly reduced(P<0.05)and the expression levels of RASSF1A and DOK7 mRNA significantly increased(P<0.05).Our findings reveal for the first time the impact of DSCR1 ectopic expression on the methylation status of breast cancer cells and identify a novel agent for epigenetic therapy.
推荐文章
DSCR1抗体制备与应用
阿尔茨海默病
DSCRI
免疫沉淀
泛素化
唐氏综合症决定域蛋白1泛素化分析
阿尔茨海默病
DSCR1
免疫沉淀
泛素化
Determination of brominated diphenyl ethers in atmospheric particulate matter using selective pressu
Brominated diphenyl ethers
Atmospheric particulate matters
Selective pressurised liquid extraction
Gas chromatography-mass spectrometry
内容分析
关键词云
关键词热度
相关文献总数  
(/次)
(/年)
文献信息
篇名 Epigenetic Modulations Induction Using DSCR1 Ectopic Expression in Breast Cancer Cells
来源期刊 分子与细胞生物力学(英文) 学科 医学
关键词 BREAST cancer METHYLATION DSCR1 REP PCR
年,卷(期) 2019,(1) 所属期刊栏目
研究方向 页码范围 41-58
页数 18页 分类号 R73
字数 语种
DOI
五维指标
传播情况
(/次)
(/年)
引文网络
引文网络
二级参考文献  (0)
共引文献  (0)
参考文献  (0)
节点文献
引证文献  (0)
同被引文献  (0)
二级引证文献  (0)
2019(0)
  • 参考文献(0)
  • 二级参考文献(0)
  • 引证文献(0)
  • 二级引证文献(0)
研究主题发展历程
节点文献
BREAST
cancer
METHYLATION
DSCR1
REP
PCR
研究起点
研究来源
研究分支
研究去脉
引文网络交叉学科
相关学者/机构
期刊影响力
分子与细胞生物力学(英文)
季刊
1556-5297
江苏省南京市浦口区东大路2号东大科技园A
出版文献量(篇)
27
总下载数(次)
0
总被引数(次)
0
论文1v1指导