Isolation and Characterization of Bacterium isolated from Bantala Tannery Solid Wastes

• 作者: Anannya Banerjee Sudipta Tripathi
• 来源期刊: NASS农业科学杂志
• 学科: 工学
• 关键词: Chromium tolerant bacterium MIC Growth curve 16S rDNA Phylogenetic analysis SEM EDX
• 年，卷（期）: 2019,（1）
• 页码范围: 26-31
• 页数: 6页
• 分类号: TG1

摘要

Leather industries that uses the conventional chrome tanning process are subjected to high risk of contamination due to the emission of toxic Cr(VI)that poses a serious threat to the environment and human’s wellbeing.The present study were made to isolate and characterize chromium tolerant bacteria in the samples collected from four different plots of Bantala Tannery,Kolkata,West Bengal,India.Pure chromium tolerant bacterial strains were isolated from the tannery sludge samples and their relative MIC(Minimum Inhibitory Concentration)were recorded at different concentrations of Cr(VI)salts to select the highest chromium tolerant bacterium.The selected bacterium was further taken for their growth studies followed by different cultural,morphological and molecular analysis(16S rDNA).The bacterial strain was further studied through SEM(Scanning Electron microscopy)and EDX(Energy Dispersive X-ray)spectroscopy which revealed that TW4 was a gram positive,rod shaped,endospore forming,pleomorphic bacterium with phylogenetic similarities with Isoptericola sp.and genebank accession number SUB1732465 TW4 KX640927.