Structure Prediction and Enzymatic Properties of Phytase phyS

• 作者: Bingzheng Xu Hongqing Xu Jianchun Xu Ruilian Pang Yueming Li
• 来源期刊: 酶研究进展(英文)
• 学科: 生物学
• 关键词: STRUCTURAL PREDICTION ENZYMATIC PROPERTIES PHYTASE
• 年，卷（期）: 2019,（4）
• 页码范围: 57-65
• 页数: 9页
• 分类号: Q55

摘要

Phytase is a kind of enzyme that hydrolyzes phytic acid and its salts to produce inositol and phosphoric acid. As a new feed additive, phytase has great potential in animal nutrition and environmental protection. Because of its good stability, large-scale production and high activity, microbial phytase has become a hot spot in industrial application. Here, we reported the predicted structure and enzymatic properties of a phytase from Bacillus subtilis, which was named as phyS. It was clear that the optimal temperature is 35°C, and the optimal pH is 8. Meanwhile, the enzyme activity was kept at above 90% in the range of pH 8 - 9, this result demonstrated that phyS is an alkaline phytase. This study lays a foundation for the extensive application of phyS.