Ionic Liquid-Polypyrrole-Gold Composites as Enhanced Enzyme Immobilization Platforms forHydrogen Peroxide Sensing

• 作者: Haiping Su Honglai Liu Jing Wu Meng Li Yazhuo Shang Yifan He
• 来源期刊: 北京日化
• 学科: 工学
• 关键词: ionic liquids (ILs) POLYPYRROLE (PPy) GOLD particles HORSERADISH PEROXIDASE (HRP) enzyme immobilization hydrogen PEROXIDE sensor
• 年，卷（期）: 2019,（2）
• 页码范围: 15-32
• 页数: 18页
• 分类号: TQ

摘要

In this work, three different aqueous solutions containing imidazole-based ILs w让h different alkyl chain lengths ([Cnmim]Br, n = 2Z 6,12) were adopted as the medium for the synthesis of ionic liquid-polypyrrole (IL-PPy) composites. Herein, the ILs undertook the roles of the pyrrole solvent, the media for emulsion polymerization of PPy and PPy dopants, respectively. The electrochemical performances of the three IL-PPy composites on a glassy carbon electrode (GCE) were investigated by electrochemical experiments, which indicated that [Ci2mim]Br-PPy (Ci2~PPy) composites displayed better electrochemical performance due to their larger surface area and firmer immobilization on the GCE. Further, Ci2?PPy/GCE were decorated with Au microparticles by electrodeposition that can not only increase the conductivity, but also immobilize sufficient biomolecules on the electrode. Then, the obtained Ci2~PPy~Au/GCE with outstanding electrochemical performance was employed as a horseradish peroxidase (HRP) immobilization platform to fabricate a novel Ci2-PPy-Au-HRP/GCE biosensor for H2O2 detection. The results showed that the prepared Ci2-PPy-Au-HRP/GCE biosensor exhibited high sensitivity, fast response, and a wide detection range as well as low detection limit towards H2O2. This work not only provides an outstanding biomolecule immobilization matrix for the fabrication of highly sensitive biosensors, but also advances the understanding of the roles of ILs in improving the electrochemical performance of biosensors.