Effect of H 2 O 2 on Growth of Collard(Brassis oleracea L.)Seedlings Under Salt Stress

• 作者: Hongyan LI Junjie GUO Wei LI
• 来源期刊: 农业生物技术:英文版
• 学科: 农学
• 关键词: BRASSICA OLERACEA L.var.acephala f.tricolor Hort. H 2O 2 Salt stress Antioxidase Gene expression
• 年，卷（期）: 2019,（1）
• 页码范围: 43-46
• 页数: 4页
• 分类号: S

摘要

Collard variety(Brassis oleracea L.var.acephala f.tricolor Hort.)as a research material was treated with exogenous H 2O 2 and H 2O 2 scavenger dimethyl thiourea under 100 mmol/L NaCl stress.Two days later,growth rate,dry weight,fresh weight and relative water content of the plants were determined.After 6 h of treatment,the activity and gene expression of three antioxidant enzymes,superoxide dismutase(SOD),catalase(CAT)and ascorbate peroxidase(APX)in plants,were measured.The results showed that the growth rate,dry weight,fresh weight,relative water content,and the activity and gene expression of the three antioxidant enzymes in collard seedlings were higher in the treatment of salt stress with the addition of 0.05 mmol/L exogenous H 2O 2 than in the simple salt stress treatment;and when endogenous H 2O 2 was removed,the growth rate,dry weight,fresh weight,relative water content,and the activity and gene expression of the three antioxidant enzymes in plant seedlings were lower than those under simple salt stress.It is speculated that under salt stress,H 2O 2 is involved in the regulation of antioxidant defense gene expression,and it might be an important regulator of salt-induced antioxidant system in collard leaves.