Germination Characteristics and Secondary Metabolism Regulation of Scutellaria baicalensis Georgi Under Osmotic Stress with PEG

• 作者: Jia LI Jinhua LIU Qian LIU Yongqing ZHANG
• 来源期刊: 农业生物技术:英文版
• 学科: 农学
• 关键词: GERMINATION characteristics PAL C 4H SECONDARY METABOLITES
• 年，卷（期）: 2019,（1）
• 页码范围: 57-60
• 页数: 4页
• 分类号: S

摘要

[Objectives]This study was conducted to study the Germination characteristics and secondary metabolism regulation of Scutellaria baicalensis Georgi under osmotic stress.[Methods]The activity of praline,soluble sugar and protein was detected with ultraviolet spectrophotometry.The activity of phenylalanine ammonia-lyase(PAL)and cinnamic acid 4-hydroxylase(C 4H)was determined with ultraviolet spectrophotometry.The secondary metabolites were detected by high performance liquid chromatography(HPLC).[Results]The germination percentage,germination potentiality and germination index decreased at first and increased subsequently along with the enhancement of PEG concentration.The praline content,the soluble sugar content and the soluble protein content in S.baicalensis seedlings increased along with the enhancement of PEG concentration and the differences from the CK were significant(P<0.05).The PAL and C 4H in S.baicalensis seedlings decreased after osmotic stress and the differences from the CK were significant(P<0.05).The results indicate that,the scutellarin,baicalin,wogonoside,baicalein,wogonin and oroxylinA contents decreased after osmotic stress and the differences from the CK were significant(P<0.05).[Conclusions]The quality of the skullcaps could be improved by improving the activity of PAL and C 4H.