Bioactivity of compounds isolated from the leaves of the Lantana camara Linn plant

• 作者: Elida Mardiah Kartika MZ Mai Efdi Suryati Yuni Mala Sari
• 来源期刊: 中国药学（英文版）
• 学科: 医学
• 年，卷（期）: 2019,（5）
• 所属期刊栏目: 研究论文
• 页码范围: 360-368
• 页数: 9页
• 分类号: R284
• 语种: 英文

摘要

Two compounds with different bioactivities were isolated from the leaves of the Lantana camara Linn plant.Compound 1 was triterpenoid (Lantadene B) (22β-dimethylacroyloxy-3-oxoolean-12-en-28-oic-acid,C35H52O5),which was isolated from n-hexane fraction and obtained as white solid (amorphous).Compound 2 was glycoside flavonoid (5-hydroxy-6,4'-dimethoxy flavon-7-O-glucopyranose,C23H24O11),which was isolated from ethyl acetate and obtained as pale yellow solid (amorphous).Extraction was performed with maceration method using methanol solvent.Subsequently,fractionation was carried out using n-hexane and ethyl acetate solvents.Isolation and purification of both fractions were conducted using chromatography method.The structure of the isolated compounds was determined through spectral analyses of ultraviolet (UV),infrared (IR),and nuclear magnetic resonance (1H NMR and 13C NMR),distortionless enhancement by polarization transfer (DEPT),heteronuclear multiple bond connectivity (HMBC),heteronuclear single quantum correlation (HSQC),and H-H COSY (1H-1H homonuclear correlated spectroscopy).The cytotoxic activity of compound 1 was tested against MCF-7 breast cancer cells in vitro using MTT assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide),showing very strong cytotoxic activity in inhibiting the growth of MCF-7 breast cancer cells,and the IC50 value was 1.1336 μM.The antioxidant activity of compound 2 was tested using DPPH assay (1,1-diphenyl-2-picrylhydrazyl),showing strong activity in inhibiting free radicals,and its IC50 value was 71.03 mg/L.