MicroRNA Let-7 targets the ecdysone signaling pathway E75 gene to control larval-pupal development in Bactrocera dorsalis

• 来源期刊: 中国昆虫科学（英文版）
• 关键词: Bactrocera dorsalis BdE75 Bdo-Let-7 ecdysone signaling pathway
• 年，卷（期）: 2019,（2）
• 页码范围: 229-239
• 页数: 11页
• 语种: 英文
• DOI: 10.1111/1744-7917.12542

摘要

MicroRNAs (miRNAs) regulate various biological processes during insect development;however,their role in larval-pupal development in oriental fruit fly,Bactrocera dorsalis (Hendel) remains unknown.In the current study,we address the biological function of a conserved miRNA,Bdo-Let-7 in the regulation of BdE75 gene,which belongs to the ecdysone signaling pathway and participates in the larval-pupal development in B.dorsalis.Using dual luciferase reporter assay in HEK293T cells we show that Bdo-Let-7 miRNA interacts with the 3'untranslated region ofBdE75 gene and suppresses its expression.The Bdo-Let-7 and BdE75 are also co-expressed in the larval-pupal stages and in different tissues ofB.dorsalis.In in vivo experiments,the injection ofBdo-Let-7 agomir and antagomir in third instar larvae down-and up-regulated the expression of BdE75,respectively.The 20-hydroxyecdysone (20E) injection assay shows that 20E up-regulated the expression of Bdo-Let-7 on the 5th day of the larvae.Moreover,abnormal pupation and eclosion were observed after larval Bdo-Let-7 antagomir injection.Based on these results,we show that Bdo-Let-7 regulates the ecdysone signaling pathway through the exact dose of BdE75 gene,and is indispensable for normal larval-pupal development in B.dorsalis.