Characterization and functional analysis of hsp 18.3 gene in the red flour beetle, Tribolium castaneum

• 来源期刊: 中国昆虫科学（英文版）
• 关键词: development reproduction stress Tchsp18.3 Tribolium castaneum
• 年，卷（期）: 2019,（2）
• 页码范围: 263-273
• 页数: 11页
• 语种: 英文
• DOI: 10.1111/1744-7917.12543

摘要

Small heat shock proteins (sHSPs) are diverse and mainly function as molecular chaperones to protect organisms and cells from various stresses.In this study,hsp18.3,one Tribolium castaneum species-specific shsp,has been idemified.Quantitative real-time polymerase chain reaction illustrated that Tchsp18.3 is expressed in all developmemal stages,and is highly expressed at early pupal and late adult stages,while it is highly expressed in ovary and fat body at the adult period.Moreover,it was up-regulated 4532 ± 396-fold in response to enhanced heat stress but not to cold stress;meanwhile the lifespan of adults in ds-Tchsp18.3 group reduced by 15.8％ from control group under starvation.Laval RNA interference (RNAi) of Tchsp18.3 caused 86.1％ ± 4.5％ arrested pupal eclosion and revealed that Tchsp18.3 played an important role in insect development.In addition,parental RNAi of Tchsp18.3 reduced the oviposition amount by 94.7％.These results suggest that Tchsp18.3 is not only essential for the resistance to heat and starvation stress,but also is critical for normal development and reproduction in T.castaneum.