MOFzyme: Enzyme Mimics of Fe/Fe-MIL-101

• 作者: Bin Li Danhua Ma Daomei Chen Dongqi Yang Jiaqiang Wang Jingchen Zhao Liang Jiang Lingli Li Yepeng Yang Yizhou Li
• 来源期刊: 生物科学与医学(英文)
• 学科: 化学
• 关键词: METAL-ORGANIC Frameworks (MOFs) Fe-MIL-101 5% Fe/Fe-MIL-101 PEROXIDASE MIMETICS
• 年，卷（期）: 2019,（5）
• 页码范围: 213-221
• 页数: 9页
• 分类号: O6

摘要

In this work, metal-organic frameworks (MOFs) Fe-MIL-101 was synthesized by hydrothermal method, and Fe/Fe-MIL-101 with different loadings was prepared. The crystal structure of the Fe/Fe-MIL-101 sample was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and specific surface area measurement (BET). Fe/Fe-MIL-101 was found to posses an intrinsic enzyme mimicking activity similar to that found in natural horse-radish peroxidase (HRP). The Michaelis constant (Km) of 5% Fe/Fe-MIL-101 with ABTS as the substrate is about 10-fold smaller than Fe-MIL-101 and about 3-fold smaller than HRP, and about 108 times less than that of CuO NPs (Km = 10.28 mM), indicating a much higher affinity for ABTS than HRP and most of the peroxidase mimetics.