Over-Expression of MicroRNA-22 Protects Cardiomyocytes from Ischemic Injury

• 作者: Lin Ling Shaohua Gu Zicheng Ling
• 来源期刊: 心血管病(英文)
• 学科: 医学
• 关键词: MicroRNA-22 CARDIOMYOCYTE CELL ACTIVITIES
• 年，卷（期）: 2019,（10）
• 页码范围: 737-745
• 页数: 9页
• 分类号: R73

摘要

Objective: To investigate the protection effect of over-expressed MicroRNA- 22 on ischemic cardiomyocyte.?Methods: Cardiomyocytes cultured in hypoxia and ischemia condition were transfected with recombinant adenovirus vector containing microRNA-22 plus a recombinant green fluorescent protein (GFP). Transduction efficiency and expression of microRNA-22 were assessed. The expression of downstream PTEN protein expression was detected. After successful transduction, cell activities including viability, nuclei proliferation and cell apoptosis were assessed. We used CCK-8 assay to assess cell activities, EdU assay to assess nuclei proliferation and Caspase-3/LDH assay to assess cell apoptosis. Results: MicroRNA-22 was transfected successfully using adeno-virus. The expression of microRNA-22 was significantly?increased. The transduction efficiency reached more than 95%. Over-expression?of microRNA-22 incardiomyocytes down regulated PTEN protein level.?Over- expression of microRNA-22 increased cell activities, DNA proliferation and decreased cell apoptosis. Conclusion: Over-expression of microRNA-22 pro- tected cardiomyocytes from ischemic injury.