Glycosylation on proteins of the intestine and perimicrovillar membrane of Triatoma (Meccus) pallidipennis, under different feeding conditions

• 来源期刊: 中国昆虫科学（英文版）
• 关键词: feeding conditions glycoproteins lectins perimicrovillar membrane triatomine vector-parasite interactions
• 年，卷（期）: 2019,（5）
• 页码范围: 796-808
• 页数: 13页
• 语种: 英文
• DOI: 10.1111/1744-7917.12579

摘要

Trypanosoma cruzi,the causative agent of Chagas disease,interacts with molecules in the midgut of its insect vector to multiply and reach the infective stage.Many studies suggest that the parasite binds to midgut-specific glycans.We identified several glycoproteins expressed in the intestine and perimicrovillar membrane (PMM) of Triatoma (Meccus) pallidipennis under different feeding conditions.In order to assess changes in protein-linked glycans,we performed lectin and immunoblot analyses on glycoprotein extracts from these intestinal tissues using well-characterized lectins,and an antibody,which collectively recognize a wide range of different glycans epitopes.We observed that the amount and composition of proteins and glycoproteins associated with different glycans structures changed over time in the intestines and PMM under different physiological conditions.PMM extracts contained a wide variety of glycoproteins with different sugar residues,including abundant high-mannose and complex sialylated glycans.We propose that these molecules could be involved in the process of parasite-vector interactions.