Cloning of a gene-edited macaque monkey by somatic cell nuclear transfer

• 来源期刊: 国家科学评论（英文版）
• 关键词: macaque monkey cloning somatic cell nuclear transfer non-human primate models CRISPR/Cas9 gene editing circadian rhythm disorders
• 年，卷（期）: 2019,（1）
• 页码范围: 101-108
• 页数: 8页
• 语种: 英文
• DOI: 10.1093/nsr/nwz003

摘要

Cloning of macaque monkeys by somatic cell nucleus transfer (SCNT) allows the generation of monkeys with uniform genetic backgrounds that are useful for the development of non-human primate models of human diseases.Here,we report the feasibility of this approach by SCNT offibroblasts from a macaque monkey (Macacafascicularis),in which a core circadian transcription factor BMAL1 was knocked out by clustered regularly interspaced short palindromic repeat/Cas9 gene editing (see accompanying paper).Out of 325 SCNT embryos transferred into 65 surrogate monkeys,we cloned five macaque monkeys with BMAL1 mutations in both alleles without mosaicism,with nuclear genes identical to that of the frbroblast donor monkey.Further peripheral blood mRNA analysis confirmed the complete absence of the wild-type BMAL1 transcript.This study demonstrates that the SCNT approach could be used to generate cloned monkeys from fibroblasts of a young adult monkeys and paves the way for the development of macaque monkey disease models with uniform genetic backgrounds.