Dear editor,
Most of the prokaryotic cells contain a single circular chromosome.In contrast,the eukaryotic cells usually contain multiple linear chromosomes.Recently,we artificially created a single linear chromosome yeast strain SY14 from native 16 chromosomes in a haploid Saccharomyces cerevisiae,which displays minor fitness defects.1 In this study,we have created a new yeast strain which contains a single circular chromosome and apparently has not been found in nature.