Miro2 supplies a platform for Parkin translocation to damaged mitochondria

• 来源期刊: 科学通报（英文版）
• 年，卷（期）: 2019,（11）
• 页码范围: 730-747
• 页数: 18页
• 语种: 英文

摘要

PINK1/Parkin-mediated mitophagy is an important process in selective removal of damaged mitochondria,in which translocation of Parkin to damaged mitochondria is recognized as an initiation step.At present,how the damaged mitochondria are selectively recognized and targeted by Parkin is not fully understood.Here we show that Miro2,an outer mitochondrial membrane protein,undergoes demultimerization from a tetramer to a monomer and alteration in mitochondrial localization upon CCCP treatment,suggesting a CCCP-induced realignment of Miro2.The realignment of Miro2 is tightly regulated by PlNK1-mediated phosphorylation at Ser325/Ser430 and by Ca2+ binding to EF2 domain,which are both essential for the subsequent Parkin translocation.Interestingly,ablation of Miro2 in mouse causes delayed reticulocyte maturation,lactic acidosis and cardiac disorders.Furthermore,several Miro2 mutations found in the congenital lactic acidosis patients also disable its realignment and Parkin translocation.These findings reveal an important role of Miro2 to mediate Parkin translocation by sensing both depolarization and Ca2+ release from damaged mitochondria to ensure the accuracy ofmitophagy.