Inhibition of β-elemene on the expressions of HIF-lα, VEGF and i NOS in diabetic rats model

• 作者: Jun Chen Lei Chen Li-Hua Li Yan Liu Yi-Zhou Sun Yun Zhou
• 来源期刊: 国际眼科杂志:英文版
• 学科: 医学
• 关键词: Β-ELEMENE hypoxia-inducible factor-1α vascular endothelial growth factor INDUCIBLE NITRIC oxide SYNTHASE diabetic rat
• 年，卷（期）: 2019,（11）
• 页码范围: 1693-1698
• 页数: 6页
• 分类号: R73

摘要

AIM: To evaluate the effect of β-elemene on the expressions of hypoxia-inducible factor(HIF)-lα, vascular endothelial growth factor(VEGF) and inducible nitric oxide synthase(i NOS) in a streptozotocin(STZ) induced diabetic SpragueDawley(SD) rat model.METHODS: SD rats were administered an abdominal injection of STZ and induced to a diabetic model. After 6 wk course of diabetes, the treatment groups were given β-elemene through periocular and intravitreous injection separately and the control groups were given blank emulsion injection. HE staining was used to observe the morphology of retina. The m RNA expressions of HIF-1α, VEGF and i NOS was assayed by real-time polymerase chain reaction(PCR) and the protein expression was measured by Western blot and immunocytochemistry methods.RESULTS: The results indicated that the protein and m RNA expressions of HIF-1α, VEGF and i NOS after treated by β-elemene periocularly and intravitreally injections were all found to be reduced compared with the levels in the diabetic rats group(P<0.05). The inhibitory effect of intravitreal injection was more remarkable.CONCLUSION: The results show β-elemene protect the retina of diabetic rats from high glucose damage by downregulating the expression of HIF-1α, VEGF and iNOS.