Modulating gene translational control through genome editing

• 来源期刊: 国家科学评论（英文版）
• 年，卷（期）: 2019,（3）
• 页码范围: 391
• 页数: 1页
• 语种: 英文
• DOI: 10.1093/nsr/nwy123

摘要

Recent advances in genome editing have led to a wide range of applications that are not limited to the manipulation of gene function.One highly desirable application is to regulate gene expression in a targeted fashion.While gene expression is regulated at multiple levels, most research has focused on the transcriptional regulation of target genes by altering regulatory sequences, repurposing designer endonucleases to create artificial transcription factors or directly editing mRNA transcripts [1].The control of gene expression at the translational level represents another important mechanism that has not been tapped into.In a recent issue of Nature Biotechnology, Gao's group from the Chinese Academy of Sciences demonstrated a simple and potentially widely applicable approach to modulate translational control of gene expression by editing upstream open reading frames (uORFs), the short protein-coding sequences located in the 5'leader region of primary open reading frames (pORFs) [2].