God does not play dice, and neither does CRISPR/Cas9

• 来源期刊: 国家科学评论（英文版）
• 年，卷（期）: 2019,（3）
• 页码范围: 393
• 页数: 1页
• 语种: 英文
• DOI: 10.1093/nsr/nwy156

摘要

The current dogma is that Cas9-generated double-strand breaks repaired in a template-free manner lead to imprecise insertion/deletion (indel) mutations, and that only template-mediated homology-directed repair (HDR) can facilitate precise modification.Hence, the repair products of non-homologous end joining (NHEJ) are considered heterogeneous in their outcome, and generally dismissed as random by nature.With this understanding of NHEJ, it has long been used only for imprecise gene disruption, rather than deployed as a precise and predictable means for genome editing.It is also conventionally accepted that SpCas9—a variant that has been studied in extensive detail—cleaves the genomic target strand and generates blunted ends.