Cloning and Bioinformatics Analysis of FaPHYC Gene in Tall Fescue ( Festuca arundinacea )

• 作者: Degang ZHAO Lu CAI Xi CHEN Xiaoli WANG Xiaoxia LIU
• 来源期刊: 农业生物技术:英文版
• 学科: 农学
• 关键词: FESTUCA arundinacea FaPHYC GENE GENE CLONING BIOINFORMATICS analysis
• 年，卷（期）: 2019,（5）
• 页码范围: 1-5
• 页数: 5页
• 分类号: S

摘要

The cDNA of PHYC gene of tall fescue ( Festuca arundinacea ) was cloned by RT-PCR,named FaPHYC.The sequence was analyzed by bioinformatic software,and the results showed that the full-length cDNA(3816 bp) was obtained with an open reading frame predicated to be 3 411 bp,which encoded 1 136 amino acids.By BLASTP analysis,the N-terminal of FaPHYC was composed of GAF and pPhytochrome domains,and its C-terminal included two repeated PAS domains,one histidine kinase A domain and one histidine kinase-like ATPase domain.The phylogenetic analysis showed that FaPHYC was closely related to PHYCs from other Gramineae plants.The qRT-PCR results showed that FaPHYC expression was regulated by drought stress,heat stress,salt stress and nitrogen stress.The real-time quantitative PCR assay showed the expression of FaPHYC in tall fescue seedlings was continuously up-regulated during the light period and down-regulated in the dark.The results suggest that FaPHYC is photoperiod sensitive and plays a functional role in flowering regulation by photoperiod in tall fescue.The FaPHYC gene was cloned and reported from tall fescue for the first time,which lays a foundation for the investigation of the stress mechanism of FaPHYC in tall fescue plant.