Skin Anaphylaxis Test of Purified Protein HAS1 in Cavies

• 作者: Gengfeng ZHAO Guilan XING Guoru XIONG Jungang WANG Shuzhen ZHANG
• 来源期刊: 农业生物技术:英文版
• 学科: 农学
• 关键词: SACCHARUM officinarum L. BACILLUS SUBTILIS HAS Purified PROTEIN HAS1 Cavies ANAPHYLAXIS
• 年，卷（期）: 2019,（5）
• 页码范围: 73-75
• 页数: 3页
• 分类号: S

摘要

The antifungal protein HAS1 is a new antifungal protein isolated from the genome of Bacillus subtilis HAS,which has a good inhibitory effect on various pathogenic fungi in sugarcane.This study aimed at evaluating the immunological transfer reaction of the test sample through repeated skin contact by observing whether the cavy skin repeatedly exposed to purified protein HAS1 suffers from allergic reaction and how strong is the allergic reaction.The results showed that the test group and the vehicle control group exhibited no allergic reaction in the skin immediately and at 24,48 and 72 h and had an mean reaction score and a sensitization rate both of 0,so the results of the naked eye observation were both nonallergenic to cavy skin;and in the positive control group,the cavies were observed to be highly sensitized immediately after removing the drug,mildly sensitized at 24 h,and not sensitized at 48 and 72 h,which meant the skin allergy was alleviated with the observation time.It is suggested that the purified protein HAS1 is negative for cavy skin anaphylaxis test (nonallergenic to cavy skin),which provides an experimental basis for further utilization of the protein and its coding gene.