Expression and role of calcitonin gene-related peptide in mouse Aspergillus fumigatus keratitis

• 作者: Cui Li Gui-Qiu Zhao Heng-Rui Zheng Min Yin Nan Jiang Qian Wang Qiang Xu Xu-Dong Peng Yuan Wu
• 来源期刊: 国际眼科杂志:英文版
• 学科: 医学
• 关键词: CALCITONIN gene-related PEPTIDE FUNGAL KERATITIS MACROPHAGES INFLAMMATION mice
• 年，卷（期）: gjykzzywb_2019,（5）
• 页码范围: 697-704
• 页数: 8页
• 分类号: R772.21

摘要

AIM: To investigate the expression and role of calcitonin gene-related peptide(CGRP) in the mouse models induced by Aspergillus fumigatus(A. fumigatus). METHODS: C57 BL/6 mice were randomized into a control group and A. fumigatus keratitis group. The cornea photography was assessed under the slit lamp and the clinical score was recorded after infection. Western blot, real-time polymerase chain reaction(PCR) and immunohistofluorescence analysis were applied to detect CGRP expression in cornea of both groups. In vitro, tests were conducted with C57 BL/6 mice macrophages to investigate CGRP expression after interaction with A. fumigatus. Cytokines expression induced by exogenous CGRP and the antagonist CGRP8-37 in A. fumigatus-exposed macrophages was evaluated by real-time PCR and ELISA.RESULTS: The cornea expression of CGRP was significantly elevated in C57 BL/6 mice corneas and macrophages after A. fumigatus infection. After treatment with exogenous CGRP, the levels of interleukin-1β(IL-1β), tumor necrosis factor-α(TNF-α) and IL-6 were reduced, and IL-10 level was increased in the A. fumigatus stimulatedmacrophages. However, IL-1β, TNF-α and IL-6 levels were upregulated after pretreatment of CGRP8-37. But the m RNA levels of MIP-2, TGF-β and IL-10 were not changed. CONCLUSION: This study provides evidence that A. fumigatus increased CGRP expression. CGRP may play a protective role against inflammation in A. fumigatus keratitis.