Optimization of Enzymatic Extraction of Sodium Chondroitin Sulfate From Bovine Nasal Bone

• 作者: An TAN Chenzhong JIN Gaoyuan ZOU Jinmao TAN Na WU Shuanghui WANG Shunli XIAO Yihong HU Yizhi ZHU Yunyun ZHOU
• 来源期刊: 农业生物技术:英文版
• 学科: 化学
• 关键词: SODIUM CHONDROITIN sulfate TRYPSIN PHOTOMETRIC TITRATION Response surface methodology
• 年，卷（期）: 2019,（4）
• 页码范围: 186-189
• 页数: 4页
• 分类号: O62

摘要

Taking bovine nasal bone as a raw material,the sodium chondroitin sulfate was extracted by enzymolysis-ion adsorption-alcohol precipitation method,and the enzymolysis process was optimized.After single factor experiments,the response surface analysis was performed by software Design-Expert 8.0,and the optimal enzymolysis conditions were as follows:the trypsin concentration of 0.8%,the pH value of 9.0,the enzymolysis temperature at 52℃,and the enzymolysis time of 2.0 h.With this combination of conditions,the concentration of sodium chondroitin sulfate extract reached 11.3 g/L,the bovine nasal bone yield was 35%,and the finished product content was 92.5%,which provided guidance for industrial production.