Study on In-vitro Production Technology of Embryos from Young Dorper Sheep

• 作者: Hongyu GUO Huidi LUO Huihui GUO Jun LI Li ZHANG Lina XUE Wenqing DANG Yangyi MAO Zhijun WANG Zhiwu WANG
• 来源期刊: 农业生物技术:英文版
• 学科: 经济
• 关键词: YOUNG DORPER SHEEP In-vitro production technology of embryos In-vitro FERTILIZATION 2-4-cell fertilized OVUM Test-tube SHEEP
• 年，卷（期）: 2019,（6）
• 页码范围: 44-47
• 页数: 4页
• 分类号: F42

摘要

This study was conducted to investigate the in-vitro production technology of embryos from young Dorper sheep, so as to provide technical support for the utilization of ovarian follicles in young Dorper sheep. Tests were conducted from the induction of Dorper sheep of 4 to 8 weeks old using follicle stimulating hormone(FSH) and pregnant horse serum(PMSG), collection of oocytes, in-vitro oocyte maturation-fertilization-zygote cultivation and 2-4-cell-stage fertilized ovum transfer. The results showed that 585 oocytes were collected from eight Dorper sheep at the age of 4 and 8 weeks, with an average of 73.13 oocytes/sheep. 346 of the 2-4-cell-stage fertilized eggs were obtained, whose cleavage rate was 59.15%. 77 in-vitro fertilized eggs at 2-4-cell stage were transplanted into 17 recipient sheep, seven of which were pregnant and gave birth to 13 "test-tube sheep" with a conception rate of 41.18%. It is indicated that the hormone induction technique, in-vitro oocyte maturation-fertilization-zygote cultivation technique and 2-4-cell-stage fertilized ovum transfer technique used in this study can serve as effective techniques for the in-vitro production of embryos from Dorper sheep of 4-8 weeks old.