Global mapping of live cell mechanical features using PeakForce QNM AFM

• 作者: Dandan Huang Jinrong Hu Mian Long Shenbao Chen Shouqin Lü Yan Zhang
• 来源期刊: 生物物理学报:英文版
• 学科: 工学
• 关键词: Global mechanical mapping Live cell Atomic force microscopy PeakForce QNM
• 年，卷（期）: 2020,（1）
• 页码范围: 9-18
• 页数: 10页
• 分类号: TG1

摘要

Atomic force microscopy(AFM)has been widely used to acquire surface topography upon different scanning modes and to quantify mechanical properties of a cell using single-point ramp force mode.However,these traditional measurements need massive force curves originating from multiple points of a cell to exclude the potential errors resulted from limited and factitious selections of testing points,making the measurements time-consurming and highly localized.PeakForce Quantitative NanoMe-chanics(PF QNM)is a high-speed(faster by 3-4 order of magnitude)and global surface mechanical property mapping method with high spatial resolution,overcoming the drawbacks of traditional ramp mode especially used for a live cell with high heterogeneity.In this protocol,we elaborated how to run PF QNM measurements for live cells and relevant modification may be needed when extending this method to other cell-like soft materials.