Effect of Semen Extender and Storage Temperature on Motility of Ram Spermatozoa

• 作者: Joan M. Burke Mohan Acharya Rick W. Rorie
• 来源期刊: 生殖科学(英文)
• 学科: 工学
• 关键词: SEMEN EXTENDERS RAM SEMEN Liquid STORAGE SHEEP
• 年，卷（期）: szkxyw_2020,（1）
• 页码范围: 14-30
• 页数: 17页
• 分类号: TP3

摘要

Cryopreservation of ram semen results in low post-thaw survival. Thus, studies were conducted to optimize liquid-storage of chilled semen. In Experiment 1, semen collected from rams (n = 5) was extended using either milk, TRIS (tris[Hydroxymethyl]aminomethane), TEST (N-Tris (hydroxymethyl)-methyl-2-aminoethane sulfonic acid), or CJ-2 (choline-based extender) and stored at either 4℃ and 15℃. All extenders were supplemented with 5% (v/v) egg yolk. In Experiment 2, semen collected from 9 rams was distributed across TRIS or milk extenders supplemented with 5% or 20% (v/v) egg yolk, and 0 or 1% ethylene glycol (EG), during storage for 72 hours at 4℃. The effect of penicillamine, hypotaurine, and epinephrine (PHE) on motility parameters was also evaluated following storage. In Experiment 1, most of the sperm motility parameters were higher after extension, and storage at 4℃ compared with 15℃ (P P in vivo sperm viability and conception rates when using extended, stored semen for artificial insemination of ewes.