Effects of Alpinia oxyphylla on oxidative stress and expression of p47phox in diabetic nephropathy rats

• 作者: Chang Liu Kai Li Mi Li Xian Wang Yiqiang Xie
• 来源期刊: TMR现代中药
• 学科: 医学
• 关键词: ALPINIA oxyphylla DIABETIC NEPHROPATHY OXIDATIVE STRESS p47phox
• 年，卷（期）: tmrxdzy_2020,（1）
• 页码范围: 13-20
• 页数: 8页
• 分类号: R73

摘要

Objective:To explore whether Yizhi(Alpinia oxyphylla,AO)could treat diabetic nephropathy(DN)through the inhibition of p47phox expression which is related to oxidative stress.Methods:Model of DN was established in SD rats with high glucose and fat diet for 4 weeks after STZ intraperitoneal injection.The rats were randomly divided into blank group,model group,AO group and irbesartan(IRB)group.Drugs were administered for 4 weeks.Blood glucose and 24-hour urinary protein were measured regularly.The index of ROS,SOD,MDA and Nox2 were tested by ELISA and the expression of p47phox protein in renal tissues were tested by techniques of RT-PCR and Western blot.Results:The decrease of blood sugar in AO group and IRB group were not obvious(P>0.05)while the drugs could significantly reduce the amount of protein in urine(P<0.05).AO could decrease ROS,MDA and Nox2 and increase SOD(P<0.05).It also could inhibit the expression of p47phox which was increased in model group(P<0.05).The IRB group has the similar effects compared with AO group(P>0.05).Conclusion:AO could reduce the amount of 24-hour urinary protein in DN rats and the mechanism may be related to the inhibition of p47phox expression thus alleviates excessive oxidative stress response.