Antibacterial Activity of Lactic Acid Bacteria and Extraction of Bacteriocin Protein

• 作者: Md. Abu Sayeed Imran Md. Ekhlas Uddin Md. Firoz Alam Md. Khasrul Alam Md. Nur Hossain Mehadi Hasan Rony Rabiul Islam
• 来源期刊: 生命科学与技术进展(英文)
• 学科: 教育
• 关键词: LACTOBACILLUS spp. BACTERIOCIN ANTIMICROBIAL Activity SDS PAGE Biopreservative
• 年，卷（期）: 2020,（2）
• 页码范围: 49-59
• 页数: 11页
• 分类号: G63

摘要

Biopreservation systems in foods are of increasing interest for industry and consumers. Bacteriocin producing Lactobacillus spp. is considered Generally Recognize as Safe (GRAS), useful to control the fast development of pathogens and spoiling microbes in food and feed. Lactobacillus spp. was isolated from traditional winter fermented vegetable cucumber & carrot by the use of selective media. Especially De Man, Rogosa and Sharpe (MRS) Agar media were used to isolate the Lactobacillus species. Morphologically identified by gram staining & colony morphology. Biochemically recognized by catalase, oxidase, MRVP & carbohydrate fermentation test. Antimicrobial activity of Lactobacillus spp. was confirmed by Well Diffusion Method. Molecular characterization of bacteriocin protein and molecular weight determined by SDS PAGE method. The isolate was found to be facultative anaerobic, Gram positive, and catalase negative. The result of antimicrobial activity measured by the Arbitrary Unit (AU/ml) of zone of the inhibition. Six isolates found from the sample but most activities exhibited isolate 4 against Bacillus megaterium (55 mm) zone of diameter. The molecular weight of the washed bacteriocin was calculated to be about 40 kDa (Isolate 1) and 15 kDa & 30 kDa (Isolate 5). Bacteriocin protein reduces chemical preservatives and uses in future as biopreservative in food industry.