Genotypic Diversity and Characterization of Quinolone Resistant Determinants from Enterobacteriaceae in Yaoundé, Cameroon

• 作者: Agnes Bedie Eyoh Anicette Chafa Betbeui Anthony M. Smith Celine Nkenfou Emilia Enjema Lyonga Mbamyah George Mondinde Ikomey Hortense Kamga Gonsu Marie-Claire Okomo Assoumou Martha Tongo Mesembe Michel Toukam Sinata Koulla-Shiro
• 来源期刊: 医学微生物学(英文)
• 学科: 医学
• 关键词: ENTEROBACTERIACEAE Quinolone Resistance Plasmid-Mediated Quinolone Resistance qnr Genes
• 年，卷（期）: 2020,（2）
• 页码范围: 33-45
• 页数: 13页
• 分类号: R73

摘要

<strong>Background:</strong> Enterobacteriaceae causes many types of infections which are often treated with quinolones and fluoroquinolone (Q/FQ). The resistance mechanisms to Q/FQ are usually associated with mutations in the quinolone resistance determining region which alter the conformation of target amino acid residues within the protein and in the <em>qnr</em> genes. This study aimed at determining the antimicrobial resistant profile of a sample of Enterobacteriaceae from Cameroon and the genetic diversity in quinolone-resistant isolates in view of implementing a better management, treatment, control and prevention of the transmission of these resistant strains. <strong>Methods:</strong> Identification and antimicrobial susceptibility testing was done using VITEK 2. The detection of plamid-mediated quinolone resistance (PMQR) genes was carried out using the conventional PCR method. Sequencing was done using the Applied Biosystem 3500 genetic analyser. DNA fingerprint was obtained using Pulsed-Field Gel electrophoresis. <strong>Results:</strong> Among 440 Enterobacteriaceae, the most prevalent genera were: <em>Escherichia</em> 178/440 (39.5%);<em>Klebsiella</em> 148/440 (33.6%);<em>Enterobacter </em>35/440 (8%);<em>Pantoea</em> 28/440 (6.4%);<em>Proteus</em> 14/440 (3.2%) <em>Salmonella </em>13/440 (3%). Ampicillin resistance showed the highest prevalence with 371/440 (81%) and Imipenem the lowest resistance 9/440 (2.1%). The ciprofloxacin resistance rate was 161/440 (36.6%). The detected plasmid mediated quinolone resistance (PMQR) genes were: <em>qnrA</em>, 2/161 (1.2%);<em>qnrB</em>, 31/161 (19.3%);<em>qnrS</em>, 13/161 (8.1%): <em>Aac</em> (6')<em>Ib-cr</em>, 84/161 (52.2%) and <em>qepA</em>, 3/161 (1.9%). There were several mutations in the <em>parC</em> gene of <em>Klebsiella</em> leading to S80D and S80N substitutions. Two pairs of <em>Klebsiella</em> <em>peumoniae</em> strains were phenotypically and genotypically identical with 100% similarity in the dendrogramme. <strong>Conclusion:</strong> This s