Cell-free microRNA-21: biomarker for intracranial aneurysm rupture

• 作者: Jiang Yuhua Jin Hengwei Li Youxiang Liu Xinke Meng Xiangyu
• 来源期刊: 中华神经外科杂志（英文）
• 关键词: Serum miRNA-21 Intracranial aneurysm Daughter aneurysm Vascular wall remodeling
• 年，卷（期）: 2020,（3）
• 所属期刊栏目: Research
• 页码范围: 141-151
• 页数: 11页
• 语种: 中文
• DOI: 10.1186/s41016-020-00195-0

摘要

Background::Deregulation of miRNA-21 expression has been reported to be associated with vascular smooth muscle behavior and cytoskeletal stability. This study is aimed to investigate the density of serum miRNA-21 in patients with different phases of intracranial aneurysms (IAs) and explore its warning function for IA rupture.Methods::A total of 16 in 200 IA patients were selected and categorized into 4 groups based on the phase of IA. Microarray study was carried out using serum miRNA and differentially expressed miRNAs were identified. Another 24 samples from a cohort of 360 patients were added and real-time polymerase chain reaction (RT-PCR) was performed on expanded sample size ( n = 40) for miRNA-21 validation. Potential gene targets of miRNA-21 were screened out from Gene Ontology (GO) database and literatures. Results::Microarray study identified 77 miRNAs with significantly different expression levels between experimental groups and the control group. RT-PCR assays validated significant downregulation of miRNA-21 in experimental groups, among which miRNA-21 expression level of daughter aneurysm group decreased the most. Bioinformatic analyses revealed that several target genes related with miRNA-21 may be involved in IA formation and rupture.Conclusions::This study suggested that miRNA-21 had a protective effect for intracranial vascular wall against remodeling and warning function for intracranial aneurysm rupture. Significant suppression of serum miRNA-21 in IA patients may provide diagnostic clues for aneurysm rupture and guide clinical intervention.