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摘要:
Rosa rugosa is an important garden ornamental plant which belongs to the genus Rosa of the family Rosaceae. The current wild and cultivated R. rugosa are mostly purple, pink, a small amount of white, but lack of yellow, orange and so on. Flavonoids 3’-hydroxylase belongs to CYP75B subfamily of cytochrome P450, and is an essential enzyme in anthocyanins synthesis. In this experiment, RrF3’H gene was cloned from the petal of Rosa rugosa ‘Hunchun’ using RT-PCR, and bioinformatics analysis was performed. The RrF3’H gene’s full length of opening reading frame was 1687 bp, encoding 510 amino acids. The formulas of proteins encoded by RrF3’H were C2666H4149N699O734S24. The derived protein had a molecular weight of 58,506.95 Da. The aliphatic index was 90.94. It belongs to unstable hydrophilic protein. The protein consists of 46.76% α-helix, 31.04% random coil, 7.66% β-corner and 14.54% extended strand. The protein contains 21 Ser phosphorylation sites, 12 Thr phosphorylation sites, and 2 Tyr phosphorylation sites. The protein contained two O-glycosylation sites, located at positions 98 and 263 of the amino acid sequence respectively. The protein has a signal peptide site and a transmembrane structure. In addition, by comparing the expression levels of RrF3’H, we found RrF3’H was positively correlated with the depth of flower color.
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篇名 Clonging and Analysis of RrF3’ H in Rosa rugosa
来源期刊 美国分子生物学期刊(英文) 学科 化学
关键词 Clonging and ANALYSIS of RrF3’H in ROSA RUGOSA
年,卷(期) 2020,(1) 所属期刊栏目
研究方向 页码范围 51-60
页数 10页 分类号 O62
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Clonging
and
ANALYSIS
of
RrF3’H
in
ROSA
RUGOSA
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研究去脉
引文网络交叉学科
相关学者/机构
期刊影响力
美国分子生物学期刊(英文)
季刊
2161-6620
武汉市江夏区汤逊湖北路38号光谷总部空间
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191
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