Dendrobium huoshanense improves doxorubicin-induced heart failure in C57BL/6 mice

• 作者: Jihong Han Maoyun Yu Rong Huang Xiaoxiao Yang Yajun Duan Yingquan Liang Yuanli Chen Yuanyu Wang
• 来源期刊: TMR现代中药
• 学科: 医学
• 关键词: Heart failure Dendrobium huoshanense Fibrosis Apoptosis Inflammatory cytokines
• 年，卷（期）: tmrxdzy,（2）
• 页码范围: 55-65
• 页数: 11页
• 分类号: R73

摘要

Objective:To investigate the therapeutic effect of Shihu(Dendrobium huoshanense,DH)on doxorubicin(DOX)-induced heart failure in mice and the involved mechanisms.Methods:Male C57BL/6 mice were randomly divided into 3 groups:Control(Ctrl)group,DOX group and DH group.Chronic heart failure was induced by intraperitoneal injection of doxorubicin solution.Mice in DH group were fed normal chow containing DH powder for 4 weeks.After 4-week treatment,electrocardiograms were measured.At the end of experiment,serum and heart sample were collected for determination of indicators for heart failure indicators.The heart tissues were conducted HE,Masson,Sirius red staining and TUNEL staining to determine cardiac tissue morphology,fibrosis,collagen content and apoptosis,respectively.mRNA and protein expression were determined by qRT-PCR and Western blot,respectively.Results:DH reduced the DOX-induced serum biomarkers(creatine kinase,aspartate aminotransferase and lactate dehydrogenase)of heart damage and reduced heart fibrosis.Mechanically,DH inhibited myocardial apoptosis,decreased interleukin 6 and tumor necrosis factoralevels,but increased superoxide dismutase 2 expression.Conclusion:DH alleviates DOX-induced chronic heart failure by inhibiting inflammatory pathway and enhancing anti-oxidative enzymes.Our study provides the potential of DH for heart failure treatment.