A Preliminary Study in the Effect of <i>Astragali radix</i>, a Qi-Invigorating Herb, on Mitochondria: Insights at Cellular Level and Mouse Tissues Level

• 作者: Bruce Qing Tang Caixia Li Glen Rein Jie Teng Lin Wang Mengmei Li Qian Feng Xuemei Bai Yu Chen Yuming Chen Zhongzhen Cai
• 来源期刊: 中医(英文)
• 学科: 医学
• 关键词: QI Astragali radix MITOCHONDRIA Total Adenylate Antioxidant Capacity
• 年，卷（期）: zyyw_2020,（3）
• 页码范围: 113-125
• 页数: 13页
• 分类号: R28

摘要

<i><span style="font-family:Verdana;">Objectives</span></i><span style="font-family:Verdana;"></i>: To explore the effects of Qi-invigorating herbs on mitochondrial function using cultured cells and animal organs. <i></span><i><span style="font-family:Verdana;">Methods</span></i><span style="font-family:Verdana;"></i>: Using water extracts of <i></span><i><span style="font-family:Verdana;">Astragali radix</span></i><span style="font-family:Verdana;"></i>, we investigated the effect of “Qi-invigoration” on M-1 renal cells and mouse organs <i></span><i><span style="font-family:Verdana;">in-vitro</span></i><span style="font-family:Verdana;"></i> including total adenylate production (TAP), reactive oxygen species (ROS) levels, and mitochondrial membrane potential (MMP). We also examined the effect on antioxidant capacity by conducting an analysis of superoxide dismutase (SOD) and glutathione (GSH). <i></span><i><span style="font-family:Verdana;">Results</span></i><span style="font-family:Verdana;"></i>: 1) <i></span><i><span style="font-family:Verdana;">Astragali radix</span></i><span style="font-family:Verdana;"></i> increased mitochondrial TAP generation and decreased ROS levels in both mouse kidney tissues and M-1 renal cells. 2) <i></span><i><span style="font-family:Verdana;">Astragali radix</span></i><span style="font-family:Verdana;"></i> also significantly increased MMP and GSH levels in M-1 cells, but in the kidney tissue</span><span style="font-family:Verdana;">,</span><span style="font-family:""><span style="font-family:Verdana;"> there was no significant change in MMP levels and a decrease in GSH levels. 3) <i></span><i><span style="font-family:Verdana;">Astragali radix</span></i><span style="font-family:Verdana;"></i> stimulated TAP levels in the heart, spleen, lung, kidney and skeletal muscle tissue, which was accompanied by the reduction of ROS. 4) For the meridian organs that <i></span><i><span style="font-family:Verdana;">Astragali radix</span></i><span style="font-family:Verdana;"></i> belongs to