Antioxidative effect of peroxiredoxin-3 in the rat myocardium exposed to renal ischemia-reperfusion injury

• 作者: Bo Hu De-Qing Chen Guang-Tao Xu Long Xu Xin Jin Xue-Bo Li
• 来源期刊: TMR整合医学
• 学科: 医学
• 关键词: Peroxiredoxin-3 ANTIOXIDATION Renal ischemia/reperfusion-induced myocardial injury
• 年，卷（期）: tmrzhyx_2020,（10）
• 页码范围: 1-7
• 页数: 7页
• 分类号: R54

摘要

Background:Peroxiredoxin-3(Prx-3)is known to be involved in the clearance of cellular hydrogen peroxide and plays an important role in protecting the myocardial tissue against damage of oxidative stress.However,the role of Prx-3 in protecting the myocardial tissue against renal ischemia/reperfusion(I/R)-induced myocardial injury(RI/RMI)remains unknown.We aimed to examine the antioxidative effect of Prx-3 using a rat RI/RMI model in an attempt to find a new approach to the prevention and treatment of myocardial injury induced by renal I/R.Methods:A RI/RMI rat model was established to detect the renal histopathology and renal functions by hematoxylin-eosin staining staining and commercially available assay kits.The myocardial antioxidant activity and the expressions of mRNA and protein of Prx-3 in the myocardial tissue were measured by real-time polymerase chain reaction and Western blotting assay,respectively.Results:Compared with the normal and control groups,the serum level of blood urea nitrogen and serum creatinine was significantly increased in the I/R group(P<0.05).Histopathological changes in the kidneys were also more obvious,including glomerular cystic and renal interstitial hemorrhage,renal tubular epithelial cell edema,and tubular type formation.The mRNA and protein expressions of Prx-3 were increased significantly in the RI/MRI rat model as compared with those in the normal and control groups.Conclusion:Oxidative damage occurred in the remote myocardium after renal I/R and Prx-3 was able to enhance the self-regulatory ability of the myocardium against damage from oxidative stress after RI/MRI.