Composition and Molecular Weight Distribution of Albumin and Globulin Protein Isolates from Durum Wheat Genotypes

• 作者: Chong Ae Lee Daniel Hailegiorgis Faris Hailu Fikru Mekonnen Song Joong Yun
• 来源期刊: 美国植物学期刊(英文)
• 学科: 农学
• 关键词: ALBUMIN DURUM Wheat GLOBULIN POLYMORPHISM SEED Protein
• 年，卷（期）: 2020,（2）
• 页码范围: 137-147
• 页数: 11页
• 分类号: S51

摘要

This paper attempts to evaluate the banding patterns of non-gluten protein isolates from the grain of durum wheat varieties. Under reduced condition, polyacrylamide-gel electrophoresis has revealed a number of different sized albumin and globulin protein bands. The electrophoretic pattern of globulin showed more polymorphisms than that of albumin. High polymorphism, both in band intensity and occurrence, was observed between 15 kDa and 35 kDa. Most of the protein bands were scored in the range of 10 kDa and 85 kDa in the two protein fractions. At a cutoff point 2.5, cluster analysis based on the SDS-PAGE of globulin proteins classified the durum wheat varieties into three major family groups. Generally, the experiment showed the suitability and usefulness of globulin protein fractions as a genetic marker in discriminating durum wheat genotypes.