Identification and Characterization of a LEA-like Gene, CaMF5, Specifically Expressed in the Anthers of Male-fertile Capsicum annuum

• 来源期刊: 园艺学报（英文版）
• 关键词: Capsicum annuum CaMF5 Anther-specific gene Late embryogenesis abundant Expression analysis
• 年，卷（期）: 2020,（1）
• 页码范围: 39-48
• 页数: 10页
• 语种: 中文

摘要

To improve the understanding of molecular mechanisms of anther and/or pollen development in Chili pepper, in the present study, full-length cDNA and DNA sequences of the pollen development-related gene CaMF5 were obtained from the anthers of a Capsicum annuum nuclear male-fertile line. Sequence analysis indicated that the full length of CaMF5 was 747 bp, containing a maximum opening reading frame of 447 bp. Amino acid sequence alignment and phylogenetic analysis revealed that CaMF5 shared approximately 37％–77％ homology with a series of uncharacterized or hypothetical proteins and late embryogenesis abundant (LEA) proteins from other plants. However, no LEA structural domain was detected in CaMF5, which indicated that it might be a new type of LEA gene. CaMF5 was only expressed inflower buds at stages 7 and 8 and in openflowers of the male-fertile line, whereas it exhibited no expression in any examined organs of the male-sterile line. In addition, CaMF5 showed the highest transcript abundance in the anthers of the male-fertile line, with no expression being detected in any other examined organs, such as the sepals, petals, pistils, roots, stems, or leaves. Taken together, our results suggest that CaMF5 is an anther-specific gene that might encode a new type of LEA protein related to anther and/or pollen development in C. annuum.