Landuse change from native prairie to managed agriculture can have substantial impacts on soil nutrient properties. Nutrient release from soil organic matter decomposition is the soil’s inherent source of long-term fertility</span><span style="font-family:Verdana;font-size:12px;">;</span><span style="font-family:Verdana;font-size:12px;"> thus it is imperative to understand the effects of continued landuse over </span><span style="font-family:""><span style="font-size:12px;font-family:Verdana;">time to avoid mistaking actual soil property changes with simple inter-annual </span><span><span style="font-size:12px;font-family:Verdana;">variability from one year to the next. The objective of this study was to evaluate the effects of landuse (</span><i><span style="font-size:12px;font-family:Verdana;">i.e.</span></i><span style="font-size:12px;font-family:Verdana;"> managed agriculture and native prairie) in two contrasting physiographic regions (</span><i><span style="font-size:12px;font-family:Verdana;">i.e.</span></i><span style="font-size:12px;font-family:Verdana;"> the Ozark Highlands region of northwest Arkansas and the Grand Prairie region of east-central Arkansas) on the change in extractable soil nutrients over a 15-yr period from 2001 to 2016. Extractable soil Ca, Mg, and Zn increased at least two times more over time (</span><i><span style="font-size:12px;font-family:Verdana;">P</span></i><span style="font-size:12px;font-family:Verdana;"> < 0.05) under cultivated agriculture in the Grand Prairie than under native prairie in the Grand Prairie or either landuse in the Ozark Highlands. </span></span></span><span style="font-family:Verdana;font-size:12px;">Averaged across landuse</span><span style="font-family:""><span style="font-size:12px;font-family:Verdana;">, extractable soil S increased nine times more over time (</span><i><span style="font-size:12px;font-family:Verdana;">P</span></i><span style="font-size:12px;font-family:Verdana;"> < 0.05) in the Ozark Highlands than in the Grand Prairie, while extract