Imbalanced dietary methionine-to-sulfur amino acid ratio can affect amino acid profiles,antioxidant capacity,and intestinal morphology of piglets

• 来源期刊: 动物营养（英文版）
• 年，卷（期）: 2020,（4）
• 所属期刊栏目: SWINE NUTRITION
• 页码范围: 447-456
• 页数: 10页
• 语种: 英文

摘要

Animal protein sources such as fishmeal and plasma powder are excellent and indispensable sources of en-ergy,amino acids,and minerals in animal production.Amino acid imbalance,especially methionine-to-sulfur amino acid(Met:SAA)ratio,caused by an imbalance of animal protein meal leads to growth restriction.This study was conducted to evaluate the effects of imbalanced Met:SAA ratio supplementation of different animal protein source diets on growth performance,plasma amino acid profiles,antioxidant capacity and intestinal morphology in a piglet model.Twenty-four weaned piglets(castrated males;BW = 10.46 ± 0.34 kg),assigned randomly into 3 groups(8 piglets/group),were fed for 28 d.Three experimental diets of equal energy and crude protein levels were as follows:1)a corn-soybean basal diet with a Met:SAA ratio at 0.51(BD);2)a plasma powder diet with a low Met:SAA ratio at 0.41(L-MR);3)a fishmeal diet with a high Met:SAA ratio at 0.61(H-MR).Results revealed that compared to BD,L-MR significantly decreased(P＜0.05)the activities of plasma total antioxidant capacity and glutathione peroxidase,plasma amino acid profiles,and significantly reduced(P＜0.05)villus height and crypt depth in the duodenum and jejunum.Additionally,L-MR signifi-cantly reduced(P＜0.05)the mRNA expression level of solute carrier family 7 member 9(SIC7A9)in the ileum,and significantly increased(P＜0.05)mRNA expression levels of zonula occludens-1(ZO-1)in the duodenum,and Claudin-1,ZO-1,sodium-coupled neutral amino acid transporters 2(SNAT2)and SIC7A7 in the jejunum.H-MR significantly increased(P＜0.05)plasma SAA levels,and significantly reduced(P＜0.05)average daily feed intake,villus height,and villus height-to-crypt depth(VH∶CD)ratio in the ileum compared to BD.In conclusion,L-MR may result in oxidative stress and villous atrophy but proves beneficial in improving in-testinal barrier function and the activity of amino acid transporters for compensatory growth.H-MR may impair intestinal growth and development for weaned piglets.The research provides a guidance on the adequate Met:SAA ratio(0.51)supplementation in diet structure for weaned piglets.