The Ozark Highlands is a unique botanical transition zone where native prairie and forest once co-existed, but conversion to managed agricultural land</span><span style="font-size:12px;font-family:Verdana;">use has severely reduced the extent of native tallgrass prairie. Quantifying soil nutrient changes over time can contribute to improved understan</span><span style="font-size:12px;font-family:Verdana;">ding of the importance of soil fertility in prairie restoration success. The objective of t</span><span><span style="font-size:12px;font-family:Verdana;">his study was to evaluate the effects of prairie ecosystem [</span><i><span style="font-size:12px;font-family:Verdana;">i.e.</span></i><span style="font-size:12px;font-family:Verdana;">, chronose</span></span><span style="font-size:12px;font-family:Verdana;">quence of four prairie restorations and a native prairie (NP)] and soil moisture regime (SMR;aquic and udic) on the change in extractable soil nutrients </span><span><span style="font-size:12px;font-family:Verdana;">over a 12-yr period from 2005 to 2017 in the Ozark Highlands region of northwest Arkansas. Soil Ca content decreased over time (</span><i><span style="font-size:12px;font-family:Verdana;">P</span></i><span style="font-size:12px;font-family:Verdana;"> ≤ 0.05</span></span><span style="font-size:12px;font-family:Verdana;">) in the 17-year-old-aquic and NP-udic combinations, which did not differ and averag</span><span style="font-size:12px;font-family:Verdana;">ed </span></span><span style="font-family:""><span style="font-size:12px;font-family:Verdana;">?</span><span><span style="font-size:12px;font-family:Verdana;">55.7 kg?ha</span><sup><span style="font-size:12px;font-family:Verdana;">?1</span></sup><span style="font-size:12px;font-family:Verdana;">?yr</span><sup><span style="font-size:12px;font-family:Verdana;">?1</span></sup><span style="font-size:12px;font-family:Verdana;">, but did not change over time in all other ecos</span></span></span><span style="font-family:""><span style="font-size: