The core of the natural product chemistry laboratory is isolation secondary metabolit</span><span style="font-family:Verdana;">e</span><span style="font-family:""><span style="font-family:Verdana;">s. One of the potential secondary metabolites for isolation in the natural product chemistry laboratory is routine (quercetin-3-O-glycosides). Routine (Quercetin-3-O-glycoside) has been isolated from ethanol extract of rubber cassava leaves (</span><i><span style="font-family:Verdana;">Manihot glaziovii </span></i><span style="font-family:Verdana;">MA). Isolation was done by maceration and recrystallization. The isolation method used in this study is complemented by the isolation method published. The isolated (Quercetin-3-O-glycoside) routine using this method obtained a yield of 0.118% of the total dried leaf extract. The routine (Quercetin-3-O-glycoside) was identified using a standard routine. Routine can be further utilized in the world of medicine as an amplifier of capillary structure, reducing the permeability and fragility of blood vessels.