Preservation of Raw Camel Milk by Lactoperoxidase System Using Hydrogen Peroxide Producing Lactic Acid Bacteria

• 作者: Adane Shegaw Amsalu Waktola Dakalo Dashe Egon Bech Hansen Mitiku Eshetu Mohammed Yusuf Kurtu Tesfemariam Berhe Yonas Hailu
• 来源期刊: 动物科学期刊(英文)
• 学科: 数学
• 关键词: Camel Milk Lactoperoxidase System Lactic Acid Bacteria PRESERVATION
• 年，卷（期）: 2020,（3）
• 页码范围: 387-401
• 页数: 15页
• 分类号: O17

摘要

This study was conducted to investigate the effect of lactic acid bacteria (LAB) activated lactoperoxidase system (LPs) on keeping quality of raw camel milk at room temperature. Camel milk samples were collected from Errer valley, Babile district of eastern Ethiopia. The level of hydrogen peroxide (H₂O₂) for activation of LPs was optimized using different levels of exogenous H₂O₂. Strains of LAB (<span style="white-space:nowrap;"><i></span>Lactococcus lactis 22333<span style="white-space:nowrap;"></i></span>, <span style="white-space:nowrap;"><i></span>Weissella confusa<span style="white-space:nowrap;"></i></span> 22308, <span style="white-space:nowrap;"><i></span>W. confusa<span style="white-space:nowrap;"></i></span> 22282, <span style="white-space:nowrap;"><i></span>W. confusa<span style="white-space:nowrap;"></i></span> 22296, <span style="white-space:nowrap;"><i></span>S. Infatarius<span style="white-space:nowrap;"></i></span> 22279 and <span style="white-space:nowrap;"><i></span>S. lutetiensis<span style="white-space:nowrap;"></i></span> 22319) with H₂O₂ producing properties were evaluated, and <i>W. confusa</i> 22282 was selected as the best strain to produce H₂O₂. Storage stability of the milk samples was evaluated through the acidification curves, titratable acidity (TA), total bacterial count (TBC) and coliform counts (CC) at storage times of 0, 6, 12, 18, 24 and 48 hours. The LP activity and the inhibitory effect of activated LPs were evaluated by growing <span style="white-space:normal;"><i></span>E. coli<span style="white-space:normal;"></i></span> in pasteurized and boiled camel milk samples as contaminating agent. Results indicated that the <span style="white-space:normal;"><i></span>W. confusa<span style="white-space:normal;"></i></span> 22282 activated LPs generally showed significantly (P < 0.05) slower rates of acidification, lactic acid production and lower TBC and CC during the storage time compared to the