Differentiation of Human Adipose-Derived Stem Cells into Endometrial Epithelial Cells

• 作者: Chen Shu-Qiang Lu Jie Sun Hui-Jun Wang Xiao-Hong Xiao Xi-Feng Yang Fang Zhang Wan-Lin
• 来源期刊: 生殖与发育医学（英文）
• 关键词: Adipose-derived Stem Cell Endometrial Epithelial Cell Estrogen Receptor α Estrogen Receptor β H19 β-catenin
• 年，卷（期）: 2020,（3）
• 所属期刊栏目: Original Article
• 页码范围: 137-145
• 页数: 9页
• 语种: 中文
• DOI: 10.4103/2096-2924.296547

摘要

Objective::This study aimed to investigate the differentiation of human adipose-derived stem cells (hASCs) into endometrial epithelial cells (EECs) under certain induction conditions and to a further step provide a promising approach for ASCs in clinical practice to the treatment of severe intrauterine adhesion.Methods::Four groups of hASCs were separately cultured as follows: in Group 1, hASCs were cultured in a control medium (5% fetal bovine serum [FBS] + α-minimum Eagle’s medium [α-MEM]); in Group 2, hASCs were cultured in an induction medium (5% FBS + α-MEM + [1 × 10 -7 mol/L 17β-estradiol] + 10 ng/mL transforming growth factor β1 [TGF-β1] + 10 ng/mL epidermal growth factor [EGF] + 10 ng/mL platelet-derived growth factor BB [PDGF-BB]); in Group 3, hASCs and human endometrium cells (hEMCs) were cocultured in the control medium; and in Group 4, hASCs and hEMCs were cocultured in the induction medium. Results::When cocultured with hEMCs, the morphology of hASCs became similar with EECs, and the addition of factors such as EGF, TGFβ, PDGF-BB, and 17β-estradiol promoted differentiation. This study, for the first time, demonstrated estrogen receptor (ER)α and ERβ expression in hASCs and preliminarily explored changes in ERα, ERβ, β-catenin, and H19 mRNA expression during hASC differentiation. Furthermore, we concluded that H19 mRNA expression was negatively correlated with differentiation, which is seemingly related to the estrogen signaling pathway.Conclusions::hASCs revealed the potential for differentiating to EECs when cocultured with hEMCs.