The Influence of Perivascular Adipose Tissue on Vascular Function in a Rabbit Model

• 作者: Abdulqader Abdulrazzaq Simon Kennedy
• 来源期刊: 药理与制药(英文)
• 学科: 数学
• 关键词: Perivascular Adipose Tissue Nitric Oxide MYOGRAPHY
• 年，卷（期）: 2020,（12）
• 页码范围: 331-349
• 页数: 19页
• 分类号: O17

摘要

<span style="font-family:Verdana;">The recent acknowledgment of the paracrine role of perivascular adipose tissue (PVAT) in vascular modulation has been supported by many studies investigating major arteries in several animal models and humans. The influence of PVAT on the functional activity of the vascular bed has been a matter of debate, whether it is an anticontractile effect with protective roles or a </span><span style="font-family:Verdana;">pro-contractile effect, investigations are underway to address this obscuri</span><span style="font-family:Verdana;">ty. In this investigation</span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> we have studied the effects of vasoconstrictors,</span><span style="font-family:Verdana;"> phen</span><span style="font-family:""><span style="font-family:Verdana;">y</span><span style="font-family:Verdana;">lephrine and noradrenaline, and vasorelaxants, carbachol and s-nitroso-n-</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">acetylpenicillamine (SNAP), on subclavian and iliac rings with and without PVAT attached;and concentration-response curves were constructed accordingly. Levels of nitric oxide (NO) generated due to activation of the enzyme adenosine monophosphate-activated protein kinase (AMPK) by 5-Aminoimi</span><span style="font-family:Verdana;">- </span><span style="font-family:""><span style="font-family:Verdana;">dazole-4-carboxamide-1-</span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;">-D-ribofuranoside (AICAR) were measured in sub</span></span><span style="font-family:Verdana;">- </span><span style="font-family:""><span style="font-family:Verdana;">clavian and iliac PVAT conditioned media. Additionally, Haematoxylin and Eosin staining was performed to analyze and compare the histological characteristics of both arteries. Subclavian and iliac rings with PVAT attached showed stronger contraction to phenylephrine and noradrenaline than that of </span><span st