Recent advances and application in whole-genome multiple displacement amplification

• 作者: Naiyun Long Yi Qiao Zheyun Xu Jing Tu Zuhong Lu
• 来源期刊: 定量生物学（英文版）
• 年，卷（期）: 2020,（4）
• 所属期刊栏目: REVIEW
• 页码范围: 279-294
• 页数: 16页
• 语种: 英文

摘要

Background:The extremely small amount of DNA in a cell makes it difficult to study the whole genome of single cells,so whole-genome amplification(WGA)is necessary to increase the DNA amount and enable downstream analyses.Multiple displacement amplification(MDA)is the most widely used WGA technique.Results:Compared with amplification methods based on PCR and other methods,MDA renders high-quality DNA products and better genome coverage by using phi29 DNA polymerase.Moreover,recently developed advanced MDA technologies such as microreactor MDA,emulsion MDA,and micro-channel MDA have improved amplification uniformity.Additionally,the development of other novel methods such as TruePrime WGA allows for amplification without primers.Conclusion:Here,we reviewed a selection of recently developed MDA methods,their advantages over other WGA methods,and improved MDA-based technologies,followed by a discussion of future perspectives.With the continuous development of MDA and the successive update of detection technologies,MDA will be applied in increasingly more fields and provide a solid foundation for scientific research.