Screening and Identification of Efficient Cellulose-degrading Strain and Its Preliminary Application in Sheep Dung Compost

• 作者: Sun Yuanfeng Zhong Jie Zheng Juan Wu Yongjun
• 来源期刊: 动物与饲料科学:英文版
• 学科: 农学
• 关键词: Sheep dung STRAIN Cellulose-degrading strain IDENTIFICATION Cellulase activity
• 年，卷（期）: 2020,（5）
• 页码范围: 1-6
• 页数: 6页
• 分类号: S14

摘要

The paper was to solve the problems of incomplete fermentation and more cellulose impurities in finished products caused by high cellulose content in sheep dung and accessory substances in composting production.[Method]Efficient cellulose-degrading strains were screened from different animal dung samples and three widely used commercial microbial composting strains(RW,BDM and JBB).The target strains were comprehensively screened by Congo red staining,degradation test of filter strips and cellulase activity test,and the strains screened were identified by physiological and biochemical tests and molecular tests.The strain was used to produce bioaugmentation strain,which was compared with composting fermentation of commercial strains.The temperature of composting process and the physical and chemical properties of finished products were tested to determine whether it met the national standards.[Result]A strain(M2)with strong cellulose-degrading ability from horse dung was screened,and its cellulase activity was(126.06±0.36)U/mL,higher than that of cellulose strain with the highest enzyme activity in commercial strains.Through morphological,physiological and biochemical identification and 16S rDNA sequence analysis,strain M2 was identified as Sphingomonas paucimobilis belonging to Sphingomonas sp.Compared with commercial strains,the bioaugmentation strain effectively improved the cellulose-degrading ability of strains after composting fermentation.The products were uniform in color,without obvious cellulose impurities,and the total nutrients were improved to a certain extent.[Conclusion]The strain M2 screened can be used for the development of specific strain for sheep dung composting.