Fermentative Biohydrogen Production with Enteric Bacteria Isolated from the Intestine of Wild Common Carp Dwelling in Tarim River Basin

• 作者: Hui Jiang Qin Zhang Yanbin Li
• 来源期刊: 生命科学与技术进展(英文)
• 学科: 经济
• 关键词: Fermentative Biohydrogen Production Enteric Bacteria INTESTINE Cotton Stalk Hydrolysate Wild Common Carp
• 年，卷（期）: 2020,（9）
• 页码范围: 442-456
• 页数: 15页
• 分类号: F42

摘要

The biological hydrogen generating from fermentation of low-cost lignocellulosic feedstocks by hydrogen-producing bacteria has attracted many attentions in recent years. In the present investigation, ten hydrogen-producing bacteria were newly isolated from the intestine of wild common carp (</span><span style="font-family:Verdana;"><i>Cyprinus carpio</i></span><span style="font-family:Verdana;"> L.), and identified belonging to the genera of </span><i><span style="font-family:Verdana;">Enterobacter</span></i><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">Klebsiella</span></i><span style="font-family:Verdana;"> based on analysis of the 16S rDNA gene sequence and examination of the physiological and biochemical characteristics. All the isolates inherently owned the ability to metabolize xylose especially the cotton stalk hydrolysate for hydrogen production with hydrogen yield (HY) higher than 100 mL</span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;">&middot;</span></span><span></span><span></span><span style="font-family:""><span style="font-family:Verdana;">L</span>^{<span style="font-family:Verdana;">-1</span>}<span style="font-family:Verdana;">. In particular, two isolates, WL1306 and WL1305 obtained higher HY, hydrogen production rate (HPR), and hydrogen production potential (HPP) using cotton stalk hydrolysate as sugar substrate than the mixed sugar of glucose & xylose, which obtained the HY of 249.5 ± 29.0, 397.0 ± 36.7 mL</span></span><span style="font-family:Verdana;">&middot;</span><span style="font-family:Verdana;">L</span>^{<span style="font-family:Verdana;">-</span>}^{<span style="font-family:Verdana;">1</span>}<span style="font-family:Verdana;">, HPR of 10.4 ± 1.2, 16.5 ± 1.5 mL</span><span style="font-family:Verdana;">&middot;</span><span style="font-family:Verdana;">L</span>^{<span style="font-family:Verdana;">-</span>}^{<span style="font-family:Verdana;">1</span>}<span style="font-family