The protective effects of Gastrodia elata Bl.decoction and Gastrodin on Acrylamide-induced DNA damage in mice

• 作者: Chunfu Wu Fan Wang Jingyu Yang Kuo Zhang Xiaoya Du
• 来源期刊: 亚洲传统医药
• 学科: 化学
• 关键词: DNA damage Gastrodia elata Bl.decoction GASTRODIN acrylamide oxidative stress
• 年，卷（期）: 2020,（6）
• 页码范围: 299-308
• 页数: 10页
• 分类号: O62

摘要

Acrylamide(AA)is ubiquitous in packaging materials,paints,cosmetics,food and drinking water.However,AA has obvious neurotoxicity,reproductive toxicity and carcinogenicity,which seriously endangers human health.As one of precious traditional Chinese medicines,Gastrodia elata Bl.has been mainly used to lower blood pressure and resist myocardial ischemia.However,the antioxidant activity of Gastrodia elata Bl.on AA-induced DNA damage has not been reported.We found that AA(25,30 and 50 mg/kg)could cause DNA damage and the damage was time/dose dependent.After AA administration,Tail moment,micronuclei formation rate,8-OHdG and MDA level were significantly increased and SOD activity was decreased.Gastrodia elata Bl.decoction,gastrodin and vitamin C could reduce AA-induced DNA damage by decreasing AA-induced increment of tail moment and micronuclei formation rate.What’s more,Gastrodia elata Bl.decoction,gastrodin and vitamin C could lower 8-OHdG and MDA level and improve SOD activity.In conclusion,our research showed that Gastrodia elata Bl.decoction and gastrodin had protective effects on AA-induced DNA damage,and the potential protective mechanism might be related to anti-oxidative stress.