Screening and Characterization of Antibiotic Producing Organisms from Waste Dump Soil Sample

• 作者: Chigoziri T. Ogu Chinenye U. Ezebialu Ebere M. Eze Ikechukwu B. Awuribeh Ruth Asikiya Afunwa Uchechukwu G. Nwankwo
• 来源期刊: 微生物学(英文)
• 学科: 经济
• 关键词: ANTIBIOTICS Crude Extract CIPROFLOXACIN Soil Sample
• 年，卷（期）: 2020,（9）
• 页码范围: 422-433
• 页数: 12页
• 分类号: F42

摘要

This study was designed to screen and characterize antibiotic producing bacteria and fungi from soil samples from a waste dump site in Enugu. The molecular characterization of the isolates was performed by using Zymo Research Quick-DNA fungi/bacteria miniprep kit. The bacteria isolates were <i>Providencia stuartii</i>, <i>Providencia alcalifaciens.</i> <i>Bacillus sp.</i>, <i>Streptococcus sp.</i>, <i>Micrococcus sp.</i>, and <i>Staphylococcus sp</i> while <i>Aspergillus fumigatus</i> and <i>Aspergillus niger</i> were fungal isolates. The efficacy of the Crude Extract (CE) of the antibiotics produced was tested against species of <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>. The CE of antibiotics when compared with a conventional antibiotic (Ciprofloxacin) showed lower antimicrobial activity. The minimum inhibitory concentration of the crude extracts ranged between 12.5% and 25% concentrations while the minimum bactericidal concentration ranged from 25% and 50% concentrations. The findings suggest that these organisms have the potential to produce antibiotics and could be harnessed by pharmaceutical industries for the production of newer antibiotics.