[Objective]The paper was to analyze the clinical therapeutic effects of Chinese herbal medicine on pathogenic bacteria of cow mastitis.[Method]Eight Chinese herbs and three compound extracts were selected for the treatment of cow mastitis.Four kinds of pathogenic bacteria were successfully isolated from cows with mastitis by flat perforation irrigation method and tube double dilution method.In addition,in vitro antibacterial test was carried out,and in vitro inhibitory effects of single and compound Chinese medicine extracts on pathogenic bacteria causing cow mastitis were studied.[Result]A total of seven Chinese herbs had good inhibitory effect,such as Taraxacum mongolicum,Viola yedonensis and Polygala japonica,which received good effect of activating blood circulation and removing blood stasis,clearing away heat and toxic materials.[Conclusion]As good Chinese herbal medicines,T.mongolicum,V.yedonensis and P.japonica could be popularized.